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Oxford Nanopore basecall quality scores

A recent switch in Oxford Nanopore basecaller software (albacore v1.0.1) substantially improved the per-base quality scores, as mentioned in a previous post. I wondered if those quality scores are accurate. As shown below, the average base quality of a read is above 16. These scores are Phred-scaled quality scores, meaning they correspond to the -10*log10(Probability of incorrect… Continue reading Oxford Nanopore basecall quality scores